{"id":594,"date":"2018-09-26T21:00:54","date_gmt":"2018-09-26T19:00:54","guid":{"rendered":"http:\/\/mttlab.eu\/?page_id=594"},"modified":"2018-09-26T21:01:25","modified_gmt":"2018-09-26T19:01:25","slug":"s9-stability-assay","status":"publish","type":"page","link":"https:\/\/mttlab.eu\/index.php\/in-vitro-services\/s9-stability-assay\/","title":{"rendered":"S9 Stability Assay"},"content":{"rendered":"<p><strong>S9 fraction Stability Assay <\/strong>is very well suited to obtain an early knowledge about the metabolism of a new chemical entity. This is valuable in the drug development process by providing essential information for the selection of leads from among a number of compounds having a similar therapeutic potential. Early in vitro metabolism studies can be used to rank ordering drug hits\/leads, and provide an insight into possible interspecies differences in toxicity and the therapeutic efficacy of the selected compounds.<\/p>\n<p>The so called S9 fraction of liver homogenate contains the vast majority of both membrane-bound and soluble phase 1 (i.e. oxidative, reductive and hydrolytic) and phase 2 (i.e. conjugative) enzymes involved in drug metabolism. Thus, the S9 stability assay, compared to our microsomal stability assay, more broadly assesses the overall hepatic metabolism of the test compound.<\/p>\n<p>Our standard S9 stability assay examines the disappearance over time of a test compound in incubations mixtures containing the liver S9 fraction, both in the presence and absence of the cofactors needed for CYP-mediated drug oxidations (i.e., <em>nicotinamide adenine dinucleotide phosphate<\/em>; NADPH) and glucuronidations (i.e., uridine diphosphoglucuronic acid; UDPGA). S9 fractions from a variety of animal species can be used to predict interspecies differences in the rate of metabolic elimination of the studied compound.<\/p>\n<p>To download the protocol example, please click <a href=\"https:\/\/mttlab.eu\/wp-content\/uploads\/2018\/09\/S9-Stability-Assay-Protocol.pdf\">here<\/a>.<\/p>\n<p><a href=\"https:\/\/i0.wp.com\/mttlab.eu\/wp-content\/uploads\/2018\/09\/S9-stability-assay-chart-2.jpg?ssl=1\"><img data-recalc-dims=\"1\" loading=\"lazy\" decoding=\"async\" data-attachment-id=\"581\" data-permalink=\"https:\/\/mttlab.eu\/index.php\/2018\/09\/27\/s9-stability-assay-in-vitro-drug-metabolism-test-for-the-selection-of-your-lead-compounds\/s9-stability-assay-chart-3\/\" data-orig-file=\"https:\/\/i0.wp.com\/mttlab.eu\/wp-content\/uploads\/2018\/09\/S9-stability-assay-chart-2.jpg?fit=838%2C491&amp;ssl=1\" data-orig-size=\"838,491\" data-comments-opened=\"0\" data-image-meta=\"{&quot;aperture&quot;:&quot;0&quot;,&quot;credit&quot;:&quot;Martin Mojzisek&quot;,&quot;camera&quot;:&quot;&quot;,&quot;caption&quot;:&quot;&quot;,&quot;created_timestamp&quot;:&quot;1537724431&quot;,&quot;copyright&quot;:&quot;&quot;,&quot;focal_length&quot;:&quot;0&quot;,&quot;iso&quot;:&quot;0&quot;,&quot;shutter_speed&quot;:&quot;0&quot;,&quot;title&quot;:&quot;&quot;,&quot;orientation&quot;:&quot;0&quot;}\" data-image-title=\"S9 stability assay chart\" data-image-description=\"\" data-image-caption=\"\" data-medium-file=\"https:\/\/i0.wp.com\/mttlab.eu\/wp-content\/uploads\/2018\/09\/S9-stability-assay-chart-2.jpg?fit=300%2C176&amp;ssl=1\" data-large-file=\"https:\/\/i0.wp.com\/mttlab.eu\/wp-content\/uploads\/2018\/09\/S9-stability-assay-chart-2.jpg?fit=838%2C491&amp;ssl=1\" class=\"alignnone size-medium wp-image-581\" src=\"https:\/\/i0.wp.com\/mttlab.eu\/wp-content\/uploads\/2018\/09\/S9-stability-assay-chart-2.jpg?resize=300%2C176&#038;ssl=1\" alt=\"\" width=\"300\" height=\"176\" srcset=\"https:\/\/i0.wp.com\/mttlab.eu\/wp-content\/uploads\/2018\/09\/S9-stability-assay-chart-2.jpg?resize=300%2C176&amp;ssl=1 300w, https:\/\/i0.wp.com\/mttlab.eu\/wp-content\/uploads\/2018\/09\/S9-stability-assay-chart-2.jpg?resize=768%2C450&amp;ssl=1 768w, https:\/\/i0.wp.com\/mttlab.eu\/wp-content\/uploads\/2018\/09\/S9-stability-assay-chart-2.jpg?w=838&amp;ssl=1 838w\" sizes=\"(max-width: 300px) 100vw, 300px\" \/><\/a><\/p>\n<p style=\"font-size: 75%;\">The diagram shows the time-dependent depletion of an alcoholic group-containing test compound by NADH*-supplemented human, rat and mouse liver S9 fraction. The data are expressed as percentage of compound remaining at each time compared with time 0 min, and represent the mean \u00b1 SD (n=3). Error bars smaller than the symbols are not visible.<br \/>\n*NADH is a cofactor for both alcohol dehydrogenase- and aldehyde dehydrogenase-dependent oxidations.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>S9 fraction Stability Assay is very well suited to obtain an early knowledge about the metabolism of a new chemical entity. This is valuable in the drug development process by providing essential information for the selection of leads from among a number of compounds having a similar therapeutic potential. Early in vitro metabolism studies can&hellip; <br \/> <a class=\"button small blue\" href=\"https:\/\/mttlab.eu\/index.php\/in-vitro-services\/s9-stability-assay\/\">Read more<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"parent":129,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"jetpack_post_was_ever_published":false,"footnotes":""},"class_list":["post-594","page","type-page","status-publish","hentry"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v25.6 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>S9 Stability Assay - MTTlab<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/mttlab.eu\/index.php\/in-vitro-services\/s9-stability-assay\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"S9 Stability Assay - MTTlab\" \/>\n<meta property=\"og:description\" content=\"S9 fraction Stability Assay is very well suited to obtain an early knowledge about the metabolism of a new chemical entity. 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