{"id":578,"date":"2018-09-27T13:00:34","date_gmt":"2018-09-27T11:00:34","guid":{"rendered":"http:\/\/mttlab.eu\/?p=578"},"modified":"2018-09-23T19:30:33","modified_gmt":"2018-09-23T17:30:33","slug":"s9-stability-assay-in-vitro-drug-metabolism-test-for-the-selection-of-your-lead-compounds","status":"publish","type":"post","link":"https:\/\/mttlab.eu\/index.php\/2018\/09\/27\/s9-stability-assay-in-vitro-drug-metabolism-test-for-the-selection-of-your-lead-compounds\/","title":{"rendered":"S9 Stability Assay – In vitro drug metabolism test for the selection of your lead compounds"},"content":{"rendered":"

MTTlab is pleased to present new \u201cin vitro drug metabolism service\u201d for the selection of your lead compounds. Our collaboration with the University of Padova allows us to offer a S9 Stability Assay for an early knowledge about the metabolism of a new chemical entity. This is valuable in the drug development process by providing essential information for the selection of leads from among a number of compounds having a similar therapeutic potential. Early in vitro metabolism studies can be used to rank ordering drug hits\/leads, and provide an insight into possible interspecies differences in toxicity and the therapeutic efficacy of the selected compounds.<\/p>\n

The so called S9 fraction of liver homogenate contains the vast majority of both membrane-bound and soluble phase 1 (i.e. oxidative, reductive and hydrolytic) and phase 2 (i.e. conjugative) enzymes involved in drug metabolism. Thus, the S9 stability assay, compared to our microsomal stability assay, more broadly assesses the overall hepatic metabolism of the test compound.<\/p>\n

Our standard S9 stability assay examines the disappearance over time of a test compound in incubations mixtures containing the liver S9 fraction, both in the presence and absence of the cofactors needed for CYP-mediated drug oxidations (i.e., nicotinamide adenine dinucleotide phosphate<\/em>; NADPH) and glucuronidations (i.e., uridine diphosphoglucuronic acid; UDPGA). S9 fractions from a variety of animal species can be used to predict interspecies differences in the rate of metabolic elimination of the studied compound.<\/p>\n

To download the protocol example, please click here<\/a>.<\/p>\n

\"\"<\/p>\n

The diagram shows the time-dependent depletion of an alcoholic group-containing test compound by NADH*-supplemented human, rat and mouse liver S9 fraction. The data are expressed as percentage of compound remaining at each time compared with time 0 min, and represent the mean \u00b1 SD (n=3). Error bars smaller than the symbols are not visible.
\n*NADH is a cofactor for both alcohol dehydrogenase- and aldehyde dehydrogenase-dependent oxidations. <\/style>\n","protected":false},"excerpt":{"rendered":"

MTTlab is pleased to present new \u201cin vitro drug metabolism service\u201d for the selection of your lead compounds. Our collaboration with the University of Padova allows us to offer a S9 Stability Assay for an early knowledge about the metabolism of a new chemical entity. This is valuable in the drug development process by providing…
Read more<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"jetpack_post_was_ever_published":false,"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_publicize_message":"","jetpack_publicize_feature_enabled":true,"jetpack_social_post_already_shared":true,"jetpack_social_options":{"image_generator_settings":{"template":"highway","enabled":false},"version":2}},"categories":[2],"tags":[],"class_list":["post-578","post","type-post","status-publish","format-standard","hentry","category-news"],"jetpack_publicize_connections":[],"yoast_head":"\nS9 Stability Assay - In vitro drug metabolism test for the selection of your lead compounds - MTTlab<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/mttlab.eu\/index.php\/2018\/09\/27\/s9-stability-assay-in-vitro-drug-metabolism-test-for-the-selection-of-your-lead-compounds\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"S9 Stability Assay - In vitro drug metabolism test for the selection of your lead compounds - MTTlab\" \/>\n<meta property=\"og:description\" content=\"MTTlab is pleased to present new \u201cin vitro drug metabolism service\u201d for the selection of your lead compounds. 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